Please describe what ‘loss-of-function’ means.
Objective 1:
You run a transcriptome analysis and find that Lis1 is a gene that is has a loss-of-function mutation more often in ASD patients compared to non-ASD controls. You would like to understand how this mutation manifests in a rodent model.
- Please describe what ‘loss-of-function’ means.
Loss-of-function means that this Lis1 gene’s product (protein) will have less to no function at all. With less function, the gene product will only be partially inactivated, but with complete loss of function it is amorphic.
- Please describe how you can examine the effects of decreased Lis1 function in mice at the RNA and protein levels.
I need help with the 2nd portion of this question. She is basically asking how you would measure changes in RNA and changes in protein, but I am not sure what the best method would be to do either one of these. Qpcr?
